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Registro Completo
Biblioteca(s): |
Embrapa Arroz e Feijão. |
Data corrente: |
15/09/2010 |
Data da última atualização: |
08/01/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
B - 1 |
Autoria: |
GONDIM, S. G. C. A.; RESENDE, L. V.; BRONDANI, R. P. V.; COLLEVATTI, R. G.; SILVA-JÚNIOR, N. J.; PEREIRA, R. R.; TELLES, M. P. C. |
Afiliação: |
S. G. C. A. GONDIM, UFG; L. V. RESENDE, UFG; ROSANA PEREIRA VIANELLO BRONDANI, CNPAF; R. G. COLLEVATTI, UFG; N. J. SILVA-JÚNIOR, SYSTEMA NATURAE CONSULTORIA AMBIENTAL, Goiânia; R. R. PEREIRA, UFG; M. P. C. TELLES, UFG. |
Título: |
Development of microsatellite markers for Hoplias malabaricus (Erythrinidae). |
Ano de publicação: |
2010 |
Fonte/Imprenta: |
Genetics and Molecular Research, v. 9, n. 3, p. 1513-1517, 2010. |
Idioma: |
Inglês |
Conteúdo: |
We identified 14 microsatellite loci for the wolf fish, Hoplias malabaricus (Erythrinidae), from a genomic shotgun library. Twenty-five primers were designed, and 48 individuals of H. malabaricus from four localities of northwest Goiás, in central Brazil, were genotyped to characterize the polymorphism at each locus. Fourteen primers amplified clearly interpretable products using a single PCR protocol; six loci were polymorphic, but with a low number of alleles per locus (2 or 3). Expected heterozygosities for polymorphic loci ranged from 0.136 to 0.505. Combined paternity exclusion probability (0.638) was low and combined genetic identity (0.056) was high in studies of parentage. The low polymorphism may be due to the small microsatellite size and the large size of the motifs. |
Palavras-Chave: |
Microssatélite. |
Thesagro: |
Cerrado; Hoplias malabaricus; Marcador molecular; Traira. |
Categoria do assunto: |
S Ciências Biológicas |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/220031/1/GMR-2010.pdf
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Marc: |
LEADER 01534naa a2200253 a 4500 001 1862333 005 2021-01-08 008 2010 bl uuuu u00u1 u #d 100 1 $aGONDIM, S. G. C. A. 245 $aDevelopment of microsatellite markers for Hoplias malabaricus (Erythrinidae).$h[electronic resource] 260 $c2010 520 $aWe identified 14 microsatellite loci for the wolf fish, Hoplias malabaricus (Erythrinidae), from a genomic shotgun library. Twenty-five primers were designed, and 48 individuals of H. malabaricus from four localities of northwest Goiás, in central Brazil, were genotyped to characterize the polymorphism at each locus. Fourteen primers amplified clearly interpretable products using a single PCR protocol; six loci were polymorphic, but with a low number of alleles per locus (2 or 3). Expected heterozygosities for polymorphic loci ranged from 0.136 to 0.505. Combined paternity exclusion probability (0.638) was low and combined genetic identity (0.056) was high in studies of parentage. The low polymorphism may be due to the small microsatellite size and the large size of the motifs. 650 $aCerrado 650 $aHoplias malabaricus 650 $aMarcador molecular 650 $aTraira 653 $aMicrossatélite 700 1 $aRESENDE, L. V. 700 1 $aBRONDANI, R. P. V. 700 1 $aCOLLEVATTI, R. G. 700 1 $aSILVA-JÚNIOR, N. J. 700 1 $aPEREIRA, R. R. 700 1 $aTELLES, M. P. C. 773 $tGenetics and Molecular Research$gv. 9, n. 3, p. 1513-1517, 2010.
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